Common Stock, par value $0.01 per share

OPKO HEALTH, INC.

4400 BISCAYNE BLVD., MIAMI, FL, 33137

Gamma Trust directly beneficially owns 216,706,448 shares of Common Stock, which constitute approximately 29.0% of the Issuer's outstanding shares of Common Stock, based upon 746,328,225 shares of Common Stock outstanding as of June 29, 2026 as communicated by the Issuer to the Reporting Person. Frost Nevada directly beneficially owns 30,127,177 shares of Common Stock, which constitute approximately 4.0% of the Issuer's outstanding shares of Common Stock, based upon 746,328,225 shares of Common Stock outstanding as of June 29, 2026 as communicated by the Issuer to the Reporting Person. Dr. Frost individually owns (i) 3,068,951 shares of Common Stock, (ii) stock options to acquire 2,425,000 shares of Common Stock, which are exercisable within 60 days of the date hereof, and (iii) 500,000 restricted stock units of which 250,000 units will vest within 60 days of the date hereof, and he has sole voting and dispositive power over each. The Phillip and Patricia Frost Philanthropic Foundation, Inc., which is controlled by Dr. Frost and his wife, owns 2,851,830 shares of Common Stock. The 255,429,406 shares of Common Stock beneficially owned by Dr. Frost constitute approximately 34.1% of the Issuer's outstanding shares of Common Stock, based upon (i) 746,328,225 shares of Common Stock outstanding as of June 29, 2026 as communicated by the Issuer to the Reporting Person; (ii) options to acquire 2,425,000 shares of Common Stock, which are exercisable within 60 days of the date hereof; and (iii) 250,000 restricted stock units that give him a contingent right to receive up to 250,000 shares of Common Stock that will vest within 60 days of the date hereof. Dr. Frost's ownership position excludes restricted stock units that give him a contingent right to receive up to 250,000 shares of Common Stock, which will not begin to vest until July, 2027.

Dr. Frost is the sole trustee of Gamma Trust and holds sole voting and dispositive power with respect to 216,706,448 shares of Common Stock held by Gamma Trust. Dr. Frost is the sole trustee of Frost Nevada and holds sole voting and dispositive power with respect to 30,127,177 shares of Common Stock owned by Frost Nevada. The Phillip and Patricia Frost Philanthropic Foundation, Inc., which is controlled by Dr. Frost and his wife, owns 2,851,830 shares of Common Stock. Dr. Frost has shared voting and dispositive power with respect to 2,851,830 shares of Common Stock owned by the Phillip and Patricia Frost Philanthropic Foundation, Inc. Dr. Frost individually owns and has sole voting and dispositive power over each of (i) 3,068,951 shares of Common Stock, (ii) stock options to acquire 2,425,000 shares of Common Stock, which are exercisable within 60 days of the date hereof, and (iii) 250,000 restricted stock units that will vest within 60 days of the date hereof. Dr. Frost's ownership position excludes restricted stock units that give him a contingent right to receive up to 250,000 shares of Common Stock, which will not begin to vest until July, 2027.

In the last 60 days prior to the filing of this Amendment No. 28, Gamma Trust, Frost Nevada and Dr. Frost have not effected any transactions in the shares of Common Stock.

Except as described herein, no other person is known by any Reporting Person to have the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, any shares of the Issuer beneficially owned by them.